Разработка метода увеличения выхода астаксантина из дрожжей Xanthophyllomyces dendrorhous
Аннотация
В рамках выпускной квалификационной работы составлен литературный обзор по физико-химическим свойствам астаксантина, его источникам и применению, продуцентам астаксантина дрожжам X. dendrorhous и биосинтезу, а также способам увеличения выхода астаксантина из дрожжей Xanthophyllomyces dendrorhous. Проведено исследование по влиянию на выход астаксантина 2,4-дихлорфеноксиуксусной кислоты в различных концентрациях, оптимальная концентрация 2,4-D для наибольшего выхода астаксантина без накопления биомассы 530 мг/л. Также найдены оптимальные концентрации необходимые для ингибирования выработки астаксантина дрожжами X. dendrorhous β-ионона (3 µмоль/л) и дифениамина (350 мкмоль/л). Выполнен химический мутагенез клеток с помощью 1-метил-3-нитро-1-нитрозогуанидина с последующим отбором чистых культур для дальнейшего изучения.
As part of the final qualification work, a literature review was compiled on the physicochemical properties of astaxanthin, its sources and applications, astaxanthin producers in yeast X. dendrorhous and biosynthesis, as well as methods for increasing the yield of astaxanthin from yeast Xanthophyllomyces dendrorhous. 4-dichlorophenoxyacetic acid in various concentrations, the optimal concentration of 2,4-D for the highest yield of astaxanthin without biomass accumulation is 5–30 mg/l. The optimal concentrations necessary for inhibiting the production of astaxanthin by the yeast X. dendrorhous of β-ionone (3 µmol/l) and dipheniamine (350 µmol/l) were also found. Chemical mutagenesis of cells was performed using 1-methyl-3-nitro-1-nitrosoguanidine followed by selection of pure cultures for further study.
As part of the final qualification work, a literature review was compiled on the physicochemical properties of astaxanthin, its sources and applications, astaxanthin producers in yeast X. dendrorhous and biosynthesis, as well as methods for increasing the yield of astaxanthin from yeast Xanthophyllomyces dendrorhous. 4-dichlorophenoxyacetic acid in various concentrations, the optimal concentration of 2,4-D for the highest yield of astaxanthin without biomass accumulation is 5–30 mg/l. The optimal concentrations necessary for inhibiting the production of astaxanthin by the yeast X. dendrorhous of β-ionone (3 µmol/l) and dipheniamine (350 µmol/l) were also found. Chemical mutagenesis of cells was performed using 1-methyl-3-nitro-1-nitrosoguanidine followed by selection of pure cultures for further study.