Получение липосомальных систем для введения флуоресцентных меток в живые клетки Preparation of liposomal systems for insertion of fluorescent labels into living cells
Аннотация
Настоящая магистерская работа посвящена синтезу перилен-1-карбальдегида реакцией перилена и 1,1-дихлорметилметилового эфира в присутствии хлорида алюминия в качестве катализатора в дихлорметане в качестве растворителя. Эти исследования в основном описывают несколько модификаций некоторых хорошо известных методологий и новых методологий. В современном органическом синтезе предпринимаются исследования для повышения эффективности реакций с использованием экологически чистых реагентов и условий реакции. Замечательными преимуществами настоящей методологии являются: отсутствие образования смеси продуктов: перилен-3-карбальдегид и перилен-1-карбальдегид, перилен-3-карбальдегид является единственным и единственным продуктом, не нужно использовать инертную атмосферу, простоту очистки и Синтез с высоким выходом. Кроме того, в качестве удлинения был также синтезирован перилен-3-илметанол с количественным выходом из перилен-3-карбальдегида. Ряд липосомальных композиций был получен путем загрузки синтезированных соединений и изучения их флуоресцентных свойств.
The present Master’s thesis is devoted to the synthesis of perylene-3-carbaldehyde by the reaction of perylene and 1,1-dichloromethyl methyl ether in presence of aluminium chloride as catalyst in dichloromethane as solvent. These investigations mainly describe a few modifications of some well-known methodologies and new methodologies. In modern organic synthesis, research efforts are being made to enhance efficiency of reactions using environmental friendly reagent and reaction conditions. The remarkable advantages of the present methodology are: no formation of mixture of products: perylene-3-carbaldehyde & perylene-1-carbaldehyde, perylene-3-carbaldehyde is the one and only product, no need to use inert atmosphere, simple purification and high yield synthesis. In addition, as an extension, perylen-3-ylmethanol was also synthesized with quantitative yield from the perylene-3-carbaldehyde. A number of liposomal compositions were obtained by loading with the synthesized compounds and studied their fluorescent properties.
The present Master’s thesis is devoted to the synthesis of perylene-3-carbaldehyde by the reaction of perylene and 1,1-dichloromethyl methyl ether in presence of aluminium chloride as catalyst in dichloromethane as solvent. These investigations mainly describe a few modifications of some well-known methodologies and new methodologies. In modern organic synthesis, research efforts are being made to enhance efficiency of reactions using environmental friendly reagent and reaction conditions. The remarkable advantages of the present methodology are: no formation of mixture of products: perylene-3-carbaldehyde & perylene-1-carbaldehyde, perylene-3-carbaldehyde is the one and only product, no need to use inert atmosphere, simple purification and high yield synthesis. In addition, as an extension, perylen-3-ylmethanol was also synthesized with quantitative yield from the perylene-3-carbaldehyde. A number of liposomal compositions were obtained by loading with the synthesized compounds and studied their fluorescent properties.