Получение и экстракция первичных и вторичных метаболитов из соевой мелассы Production and Extraction of Primary and Secondary Metabolites from Soy Molasses
Аннотация
Сравнивали ферментационный и традиционный методы экстракции изофлавоноидов для извлечения изофлавонов из солевой мелассы. Дайдзеин, глицитеин и генистеин были идентифицированы с помощью газовой хроматографии с масс-спектрометрией (ГХ-МС), Эксперименты по определению влияния времени экстракции на выход дайдзеина проводили с использованием метода добавки стандарта в ВЭЖХ. Для определения общего выхода изофлавоноидов и антиоксидантной способности использовали, на лабораторных животных был изучен in vivo метаболизм дайдзеина до эквола. Бактерии, образующие эквол, выделяли из фекальных образцах и культивировали анаэробно в среде Китта –Тароцци с добавлением дайдзеина для исследований in vitro. Несмотря на полный метаболизм даидзеина после 5 дней инкубации, эквол не был обнаружен.
Fermentation technique was compared to the conventional extraction method for the extraction of isoflavones from soy molasses. Daidzein, glycitein and genistein were identified by Gas chromatography equipped with mass spectrometer (GC-MS) and the effect of extraction time on daidzein yield was carried out using the High performance liquid chromatography standard addition method. The aluminum colorimetric method and DPPH radical scavenging activity was used to measure total isoflavone yield and antioxidant capacity respectively. In vivo metabolism of daidzein to equol was performed in rat and equol producing bacteria was isolated from fecal samples of rat and cultured anaerobically in kitta tarrozi medium supplemented with daidzein for in-vitro study. Although there was complete metabolism of daidzein after 5 days of incubation, equol was not identified.
Fermentation technique was compared to the conventional extraction method for the extraction of isoflavones from soy molasses. Daidzein, glycitein and genistein were identified by Gas chromatography equipped with mass spectrometer (GC-MS) and the effect of extraction time on daidzein yield was carried out using the High performance liquid chromatography standard addition method. The aluminum colorimetric method and DPPH radical scavenging activity was used to measure total isoflavone yield and antioxidant capacity respectively. In vivo metabolism of daidzein to equol was performed in rat and equol producing bacteria was isolated from fecal samples of rat and cultured anaerobically in kitta tarrozi medium supplemented with daidzein for in-vitro study. Although there was complete metabolism of daidzein after 5 days of incubation, equol was not identified.