Изучение влияния матричного эффекта на результаты исследования антиоксидантов
Аннотация
83 pages, 33 figures, 5 tables, 40 bibliographic titles
Key words: POTENTIOMETRIC METHOD, ANTIOXIDANT CAPACITY, MATRIX EFFECT, EXTRACTS OF MEDICINAL PLANTS
The object of the study is some classes of individual antioxidants: SH-containing, polyphenols and having a furan cycle in their structure, and water extracts of medicinal herbs that contain the described classes of compounds that replenish the deficiency of compounds in the body that fight free radical oxidation.
The aim of the work is to study the influence of the matrix effect on the determination of the antioxidant capacity of individual antioxidants and herbal extracts.
The study of literature data revealed that, among others, a potentiometric method for determining the antioxidant capacity is quite simple and informative. two ways of implementing this method were applied and compared: using one form of K3[Fe(CN)6] and the system K3[Fe(CN)6]/ K4[Fe(CN)6], the value of the antioxidant capacity of the research objects was determined taking into account and without taking into account the matrix, the effect of the antioxidant concentration on the experimental results was studied.
Based on the results of measurements and calculations, it was concluded that the matrix of the object influences the results of potentiometric determination of antioxidant capacity, deviating the angle of the electrode function. This effect increases with increasing concentration of the investigated object. The results obtained by the two methods are somewhat different due to the specifics of each of them.
In addition, the leading compounds were identified in providing antioxidant action among the analyzed herbal extracts.
Key words: POTENTIOMETRIC METHOD, ANTIOXIDANT CAPACITY, MATRIX EFFECT, EXTRACTS OF MEDICINAL PLANTS
The object of the study is some classes of individual antioxidants: SH-containing, polyphenols and having a furan cycle in their structure, and water extracts of medicinal herbs that contain the described classes of compounds that replenish the deficiency of compounds in the body that fight free radical oxidation.
The aim of the work is to study the influence of the matrix effect on the determination of the antioxidant capacity of individual antioxidants and herbal extracts.
The study of literature data revealed that, among others, a potentiometric method for determining the antioxidant capacity is quite simple and informative. two ways of implementing this method were applied and compared: using one form of K3[Fe(CN)6] and the system K3[Fe(CN)6]/ K4[Fe(CN)6], the value of the antioxidant capacity of the research objects was determined taking into account and without taking into account the matrix, the effect of the antioxidant concentration on the experimental results was studied.
Based on the results of measurements and calculations, it was concluded that the matrix of the object influences the results of potentiometric determination of antioxidant capacity, deviating the angle of the electrode function. This effect increases with increasing concentration of the investigated object. The results obtained by the two methods are somewhat different due to the specifics of each of them.
In addition, the leading compounds were identified in providing antioxidant action among the analyzed herbal extracts.