Хроматографическое определение бета-адреноблокаторов и блокаторов кальциевых каналов в биологических жидкостях при диагностике острых отравлений
Аннотация
The objects of the study are beta-blockers (atenolol, bisoprolol, metoprolol) and calcium channel blockers (amlodipine, nifedipine).
Objective: to develop methodological approaches to the determination of ꞵ-blockers and calcium channel blockers in biological fluids by gas chromatography with mass spectrometric detection.
The increase in acute poisoning with antihypertensive drugs is due to their availability to the population, self-medication, non-medical use and negligent storage in everyday life.
For an adequate diagnosis and provision of the necessary medical care in acute drug poisoning, it is necessary to accurately determine the cause of intoxication based on the results of chemical and toxicological analysis. In this regard, the development of methods for isolating these drugs from biological fluids, as well as methods for their chromatographic determination, is an urgent problem.
For the first time, a systematic approach to the laboratory diagnosis of acute poisoning with antihypertensive drugs is applied in this work.
Based on the literature data, the optimal conditions for gas chromatographic analysis were selected and adjusted, various methods of derivatization were evaluated.
The results of the work were tested in the analysis of urine samples of patients of the toxico-intensive care unit of the Sverdlovsk Regional Center for the Treatment of Acute poisoning.
The proposed methodological approaches for chromatographic determination of beta-blockers and calcium channel blockers in biological fluids can be used for drug therapeutic monitoring, in routine practice of chemical and toxicological laboratories for rapid diagnosis of acute poisoning.
Objective: to develop methodological approaches to the determination of ꞵ-blockers and calcium channel blockers in biological fluids by gas chromatography with mass spectrometric detection.
The increase in acute poisoning with antihypertensive drugs is due to their availability to the population, self-medication, non-medical use and negligent storage in everyday life.
For an adequate diagnosis and provision of the necessary medical care in acute drug poisoning, it is necessary to accurately determine the cause of intoxication based on the results of chemical and toxicological analysis. In this regard, the development of methods for isolating these drugs from biological fluids, as well as methods for their chromatographic determination, is an urgent problem.
For the first time, a systematic approach to the laboratory diagnosis of acute poisoning with antihypertensive drugs is applied in this work.
Based on the literature data, the optimal conditions for gas chromatographic analysis were selected and adjusted, various methods of derivatization were evaluated.
The results of the work were tested in the analysis of urine samples of patients of the toxico-intensive care unit of the Sverdlovsk Regional Center for the Treatment of Acute poisoning.
The proposed methodological approaches for chromatographic determination of beta-blockers and calcium channel blockers in biological fluids can be used for drug therapeutic monitoring, in routine practice of chemical and toxicological laboratories for rapid diagnosis of acute poisoning.